Recombinant humaan ARL5/ARL5A-eiwit

ADP-ribosylation factor-like 5A, also known ARL5A, belongs to the ARF family of GTP-binding proteins. ARFs are important in eukaryotic vesicular trafficking pathways and play an essential role in the activation of phospholipase D. unlike many ARF family members, ARL5 is thought to lack ADP-ribosylation enhancing activity. ARL5A is developmentally regulated and may play a role in nuclear dynamics and signaling cascades during embryonic development. Recombinant human ARL5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ADP-ribosylation factor-like 5A, ARFLP5, ARL5, ADP ribosylation factor like GTPase 5A, ADP-ribosylation factor-like 5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.3 kDa (203aa) confirmed by MALDI-TOF

ARL5A, ADP-ribosylation factor-like 5A, ARFLP5, ARL5, ATGP0816-10 µg, ATGP0816-20 µg, ATGP0816-50 µg, ATGP0816-100 µg, ATGP0816-250 µg, ATGP0816-500 µg, ATGP0816-1 mg, ATGP0816-10, ATGP0816-20, ATGP0816-50, ATGP0816-100, ATGP0816-250, ATGP0816-500, ATGP0816-1

Haraquchi T., et al. (2006) Biosci Biotechnol Biochem. 70:1798-1802.; ; Pasqualato S., et al. (2002) EMBO Rep. 3:1035-1041.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF

Signal Transduction

NP_036229.1

Q9Y689

Human

MGILFT RIWRLFNHQE HKVIIVGLDN AGKTTILYQF SMNEVVHTSP TIGSNVEEIV INNTRFLMWD IGGQESLRSS WNTYYTNTEF VIVVVDSTDR ERISVTREEL YKMLAHEDLR KAGLLIFANK QDVKECMTVA EISQFLKLTS IKDHQWHIQA CCALTGEGLC QGLEWMMSRL KIR