Recombinant humaan ARPP19-eiwit

CAMP-regulated phosphoprotein 19, also known as ARPP19, is protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. This protein may indirectly enhance GAP-43 expression. Recombinant human ARPP19 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CAMP-regulated phosphoprotein 19, cAMP-regulated phosphoprotein 19, ARPP-16, ARPP-19, ARPP16, ENSAL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

14.7 kDa (135aa) confirmed by MALDI-TOF

ARPP19, cAMP-regulated phosphoprotein 19, cAMP-regulated phosphoprotein 19, ARPP-16, ARPP-19, ARPP16, ENSAL, ATGP2449-10 µg, ATGP2449-20 µg, ATGP2449-50 µg, ATGP2449-100 µg, ATGP2449-250 µg, ATGP2449-500 µg, ATGP2449-1 mg, ATGP2449-10, ATGP2449-20, ATGP2449-50, ATGP2449-100, ATGP2449-250, ATGP2449-500, ATGP2449-1

Gharbi-Ayachi A., et al. (2010) Science. 330:1673-1677; ; Choudhary C., et al. (2009) Science. 325:834-840

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Signal Transduction

NP_006619

P56211

Human

MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG