Recombinant humaan Arylsulfatase A/ARSA-eiwit

ARSA hydrolyzes cerebrosidesulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. Recombinant human ARSA protein, fused to His-tag at N-terminus, was expressed in E. coli.

ASA, Cerebroside-sulfatase, metachromatic leucodystrophy (MLD)

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

54.3 kDa (512aa)

ARSA, Arylsulfatase A, MLD, ATGP2087-10 µg, ATGP2087-20 µg, ATGP2087-50 µg, ATGP2087-100 µg, ATGP2087-250 µg, ATGP2087-500 µg, ATGP2087-1 mg, ATGP2087-10, ATGP2087-20, ATGP2087-50, ATGP2087-100, ATGP2087-250, ATGP2087-500, ATGP2087-1

Matthes, F., et al. (2011) J. Biol. Chem. 286 (20), 17487-17494; ; Hayashi, T., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 105-108

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Metabolism

NP_000478.3

P15289

Human

RPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA