Recombinant humaan Arylsulfatase A/ARSA-eiwit

ARSA, also known as arylsulfatase A isoform a, is a member of the sulfatase family. It hydrolyzes cerebroside sulfate, namely cerebroside 3-sulfate. It is found in many tissues, but predominantly in myelin and kidney. This protein is activated by a posttranslational modification (PTM) with the oxidation of cysteine to formylglycine. It could be helpful in diagnosis of lung and central nervous system cancer through the serum level of this protein. The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms. Recombinant human ARSA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 2,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.

ASA, Cerebroside-sulfatase, metachromatic leucodystrophy (MLD)

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 2,500pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.

53 kDa (498aa)

ARSA, Arylsulfatase A isoform a, ARSA, ASA, MLD, ATGP3826-10 µg, ATGP3826-20 µg, ATGP3826-50 µg, ATGP3826-100 µg, ATGP3826-250 µg, ATGP3826-500 µg, ATGP3826-1 mg, ATGP3826-10, ATGP3826-20, ATGP3826-50, ATGP3826-100, ATGP3826-250, ATGP3826-500, ATGP3826-1

Shahzad MA., et al, (2017) J Mol Neurosci. 63:84-90.; ; Cesani M., et al, (2017) Hum Mutat. 37:16-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_000478

P15289

Human

Ala

RPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA