Recombinant humaan Arylsulfatase G/ARSG-eiwit

ARSG belongs to the sulfatase enzyme family. Sulfatases hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This protein displays arylsulfatase activity at acidic pH, as is typical of lysosomal sulfatases, and has been shown to localize in the lysosomes. Alternatively spliced transcript variants have been found for this gene. Recombinant human ARSG protein, fused to His-tag at N-terminus, was expressed in E. coli.

Arylsulfatase G

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

57 kDa (532aa)

ARSG, Arylsulfatase G, Arylsulfatase G, ATGP1885-10 µg, ATGP1885-20 µg, ATGP1885-50 µg, ATGP1885-100 µg, ATGP1885-250 µg, ATGP1885-500 µg, ATGP1885-1 mg, ATGP1885-10, ATGP1885-20, ATGP1885-50, ATGP1885-100, ATGP1885-250, ATGP1885-500, ATGP1885-1

Frese MA, Schulz S, et al. (2008) . J Biol Chem. 283 (17) :11388-95.; ; Hong KW, Go MJ, et al. (2010) . J Hum Hypertens. 24 (6) :367-72.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Enzymes & Proteases

NP_055775

Q96EG1

Human

MGSSHHHHHH SSGLVPRGSH MGSGFLYPLV DFCISGKTRG QKPNFVIILA DDMGWGDLGA NWAETKDTAN LDKMASEGMR FVDFHAAAST CSPSRASLLT GRLGLRNGVT RNFAVTSVGG LPLNETTLAE VLQQAGYVTG IIGKWHLGHH GSYHPNFRGF DYYFGIPYSH DMGCTDTPGY NHPPCPACPQ GDGPSRNLQR DCYTDVALPL YENLNIVEQP VNLSSLAQKY AEKATQFIQR ASTSGRPFLL YVALAHMHVP LPVTQLPAAP RGRSLYGAGL WEMDSLVGQI KDKVDHTVKE NTFLWFTGDN GPWAQKCELA GSVGPFTGFW QTRQGGSPAK QTTWEGGHRV PALAYWPGRV PVNVTSTALL SVLDIFPTVV ALAQASLPQG RRFDGVDVSE VLFGRSQPGH RVLFHPNSGA AGEFGALQTV RLERYKAFYI TGGARACDGS TGPELQHKFP LIFNLEDDTA EAVPLERGGA EYQAVLPEVR KVLADVLQDI ANDNISSADY TQDPSVTPCC NPYQIACRCQ AA