Recombinant humaan AS3MT-eiwit

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. Recombinant human AS3MT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Arsenic (+3 oxidation state) methyltransferase, CYT19, RP11-753C18.6

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

44.3 kDa (399aa) confirmed by MALDI-TOF

AS3MT, Arsenic (+3 oxidation state) methyltransferase, CYT19, RP11-753C18.6, ATGP1555-10 µg, ATGP1555-20 µg, ATGP1555-50 µg, ATGP1555-100 µg, ATGP1555-250 µg, ATGP1555-500 µg, ATGP1555-1 mg, ATGP1555-10, ATGP1555-20, ATGP1555-50, ATGP1555-100, ATGP1555-250, ATGP1555-500, ATGP1555-1

Sumi, D., et al. (2011) Biochem. Biophys. Res. Commun. 415 (1), 48-53 ; ; Watanabe, T., et al. (2011) Arch. Toxicol. 85 (6), 577-588

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Metabolism

NP_065733

Q9HBK9

Human

MAALRD AEIQKDVQTY YGQVLKRSAD LQTNGCVTTA RPVPKHIREA LQNVHEEVAL RYYGCGLVIP EHLENCWILD LGSGSGRDCY VLSQLVGEKG HVTGIDMTKG QVEVAEKYLD YHMEKYGFQA SNVTFIHGYI EKLGEAGIKN ESHDIVVSNC VINLVPDKQQ VLQEAYRVLK HGGELYFSDV YTSLELPEEI RTHKVLWGEC LGGALYWKEL AVLAQKIGFC PPRLVTANLI TIQNKELERV IGDCRFVSAT FRLFKHSKTG PTKRCQVIYN GGITGHEKEL MFDANFTFKE GEIVEVDEET AAILKNSRFA QDFLIRPIGE KLPTSGGCSA LELKDIITDP FKLAEESDSM KSRCVPDAAG GCCGTKKSC