Recombinant humaan ASB8-eiwit

Ankyrin repeat and SOCS box containing 8, also known as ASB8, may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recombinant human ASB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ankyrin repeat and SOCS box containing 8, PP14212

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.0 kDa (311aa) confirmed by MALDI-TOF

ASB8, Ankyrin repeat and SOCS box containing 8, PP14212, ATGP2341-10 µg, ATGP2341-20 µg, ATGP2341-50 µg, ATGP2341-100 µg, ATGP2341-250 µg, ATGP2341-500 µg, ATGP2341-1 mg, ATGP2341-10, ATGP2341-20, ATGP2341-50, ATGP2341-100, ATGP2341-250, ATGP2341-500, ATGP2341-1

Liu Y., et al. (2003) Biochem. Biophys. Res. Commun. 300:972-979; ; Ota T., et al. (2004) Nat. Genet. 36:40-45;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Ubiquitination

NP_077000

Q9H765

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSSMWY IMQSIQSKYS LSERLIRTIA AIRSFPHDNV EDLIRGGADV NCTHGTLKPL HCACMVSDAD CVELLLEKGA EVNALDGYNR TALHYAAEKD EACVEVLLEY GANPNALDGN RDTPLHWAAF KNNAECVRAL LESGASVNAL DYNNDTPLSW AAMKGNLESV SILLDYGAEV RVINLIGQTP ISRLVALLVR GLGTEKEDSC FELLHRAVGH FELRKNGTMP REVARDPQLC EKLTVLCSAP GTLKTLARYA VRRSLGLQYL PDAVKGLPLP ASLKEYLLLL E