Recombinant humaan ASCC1-eiwit

ASCC1 is a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF) . The protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in ASCC1 are associated with Barrett esophagus and esophageal adenocarcinoma. Recombinant human ASCC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Activating signal cointegrator 1 complex subunit 1, ASC1p50, CGI-18, p50

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.6 kDa (380aa) confirmed by MALDI-TOF

ASCC1, Activating signal cointegrator 1 complex subunit 1, ASC1p50, CGI-18, p50, ATGP2303-10 µg, ATGP2303-20 µg, ATGP2303-50 µg, ATGP2303-100 µg, ATGP2303-250 µg, ATGP2303-500 µg, ATGP2303-1 mg, ATGP2303-10, ATGP2303-20, ATGP2303-50, ATGP2303-100, ATGP2303-250, ATGP2303-500, ATGP2303-1

Almeida-Vega S., et al (2009) . Am. J. Physiol. 296:G414-G423; ; Orloff M., et al (2011) . JAMA 306:410-419

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001185727

Q8N9N2

Human

MEVLRPQ LIRIDGRNYR KNPVQEQTYQ HEEDEEDFYQ GSMECADEPC DAYEVEQTPQ GFRSTLRAPS LLYKHIVGKR GDTRKKIEME TKTSISIPKP GQDGEIVITG QHRNGVISAR TRIDVLLDTF RRKQPFTHFL AFFLNEVEVQ EGFLRFQEEV LAKCSMDHGV DSSIFQNPKK LHLTIGMLVL LSEEEIQQTC EMLQQCKEEF INDISGGKPL EVEMAGIEYM NDDPGMVDVL YAKVHMKDGS NRLQELVDRV LERFQASGLI VKEWNSVKLH ATVMNTLFRK DPNAEGRYNL YTAEGKYIFK ERESFDGRNI LKLFENFYFG SLKLNSIHIS QRFTVDSFGN YASCGQIDFS