Recombinant humaan ASF1B-eiwit

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. This protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Recombinant human ASF1B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.4 kDa (210aa) confirmed by MALDI-TOF

ASF1B, ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II, ATGP1644-10 µg, ATGP1644-20 µg, ATGP1644-50 µg, ATGP1644-100 µg, ATGP1644-250 µg, ATGP1644-500 µg, ATGP1644-1 mg, ATGP1644-10, ATGP1644-20, ATGP1644-50, ATGP1644-100, ATGP1644-250, ATGP1644-500, ATGP1644-1

Corpet, A., et al. (2011) EMBO J. 30 (3), 480-493; ; Jasencakova, Z., et al. (2010) Mol. Cell 37 (5), 736-743

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_060624.1

Q9NVP2

Human