Recombinant humaan ASGR2-eiwit

ASGR2 is a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. It may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. ASGR2 is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. ASGR2 is the less abundant minor subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human ASGR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Asialoglycoprotein receptor 2, ASGP-R2; ASGPR2; CLEC4H2; HBXBP; HL-2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.9 kDa (255aa) confirmed by MALDI-TOF

ASGR2, Asialoglycoprotein receptor 2, ASGP-R2; ASGPR2; CLEC4H2; HBXBP; HL-2, ATGP1776-10 µg, ATGP1776-20 µg, ATGP1776-50 µg, ATGP1776-100 µg, ATGP1776-250 µg, ATGP1776-500 µg, ATGP1776-1 mg, ATGP1776-10, ATGP1776-20, ATGP1776-50, ATGP1776-100, ATGP1776-250, ATGP1776-500, ATGP1776-1

Seow YY., et al. (2002) . Nephron. 91 (3) :431-8.; ; Veselkin E., et al. (2011) . PLoS One. 6 (11) :e27210.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Signal Transduction

NP_001172

P07307

Human

MGSSHHHHHH SSGLVPRGSH MGSQSEGHRG AQLQAELRSL KEAFSNFSSS TLTEVQAIST HGGSVGDKIT SLGAKLEKQQ QDLKADHDAL LFHLKHFPVD LRFVACQMEL LHSNGSQRTC CPVNWVEHQG SCYWFSHSGK AWAEAEKYCQ LENAHLVVIN SWEEQKFIVQ HTNPFNTWIG LTDSDGSWKW VDGTDYRHNY KNWAVTQPDN WHGHELGGSE DCVEVQPDGR WNDDFCLQVY RWVCEKRRNA TGEVA