Recombinant humaan ASMT-eiwit

ASMT belongs to the methyltransferase superfamily and is involved in melatonin biosynthesis. Expressed in brain, retina and pineal gland, ASMT functions to catalyze the final reaction in the synthesis of melatonin, specifically the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-L-homocysteine and melatonin. Recombinant human ASMT protein, fused to His-tag at N-terminus, was expressed in E. coli.

Acetylserotonin O-methyltransferase, HIOMT, HIOMTY

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.3 kDa (318aa)

ASMT, Acetylserotonin O-methyltransferase, HIOMT, HIOMTY, ATGP1789-10 µg, ATGP1789-20 µg, ATGP1789-50 µg, ATGP1789-100 µg, ATGP1789-250 µg, ATGP1789-500 µg, ATGP1789-1 mg, ATGP1789-10, ATGP1789-20, ATGP1789-50, ATGP1789-100, ATGP1789-250, ATGP1789-500, ATGP1789-1

Rodriguez I R., et al. (1994) J Biol Chem. 269:31969-31977.; ; Toma C., et al. (2007) Mol Psychiatry. 12:977-979.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol

Metabolism

AAH01620

P46597

Human

MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK