Recombinant humaan ASNA1-eiwit

ASNA1, also as known as ARSA and TRC40, belongs to the arsA ATPase family. ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials. ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recombinant human ASNA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ATPase ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, hASNA-I, TRC40

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.2 kDa (371aa) confirmed by MALDI-TOF

ASNA1, ATPase ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, hASNA-I, TRC40, ATGP1751-10 µg, ATGP1751-20 µg, ATGP1751-50 µg, ATGP1751-100 µg, ATGP1751-250 µg, ATGP1751-500 µg, ATGP1751-1 mg, ATGP1751-10, ATGP1751-20, ATGP1751-50, ATGP1751-100, ATGP1751-250, ATGP1751-500, ATGP1751-1

Stefanovic S., et al. (2007) Cell 128:1147-1159; ; Favaloro V., et al. (2008) J. Cell Sci. 121:1832-1840

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_004308

O43681

Human

MAAGVAG WGVEAEEFED APDVEPLEPT LSNIIEQRSL KWIFVGGKGG VGKTTCSCSL AVQLSKGRES VLIISTDPAH NISDAFDQKF SKVPTKVKGY DNLFAMEIDP SLGVAELPDE FFEEDNMLSM GKKMMQEAMS AFPGIDEAMS YAEVMRLVKG MNFSVVVFDT APTGHTLRLL NFPTIVERGL GRLMQIKNQI SPFISQMCNM LGLGDMNADQ LASKLEETLP VIRSVSEQFK DPEQTTFICV CIAEFLSLYE TERLIQELAK CKIDTHNIIV NQLVFPDPEK PCKMCEARHK IQAKYLDQME DLYEDFHIVK LPLLPHEVRG ADKVNTFSAL LLEPYKPPSA Q