Recombinant humaan ASPA-eiwit

ASPA, also known as aspartoacylase, is expressed in liver, lung and kidney tissue, as well as in skeletal muscle and in cerebral white matter. Existing as a homodimer, Aspartoacylase functions to catalyze the deacetylation of N-acetylaspartic acid (NAA) (a protein whose hydrolysis is crucial to maintenance of intact white matter) to produce acetate and L-aspartate. Recombinant human ASPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Aspartoacylase, ACY2, ASP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.1 kDa (336aa) confirmed by MALDI-TOF

ASPA, Aspartoacylase, ACY2, ASP, ATGP1421-10 µg, ATGP1421-20 µg, ATGP1421-50 µg, ATGP1421-100 µg, ATGP1421-250 µg, ATGP1421-500 µg, ATGP1421-1 mg, ATGP1421-10, ATGP1421-20, ATGP1421-50, ATGP1421-100, ATGP1421-250, ATGP1421-500, ATGP1421-1

Le Coq. et al. (2006) Biochemistry 45: 5878-5884.; ; Le Coq. et al. (2008) Biochemistry 47: 3484-3492.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF

Neuroscience

NP_000040.1

P45381

Human

MTSCHIA EEHIQKVAIF GGTHGNELTG VFLVKHWLEN GAEIQRTGLE VKPFITNPRA VKKCTRYIDC DLNRIFDLEN LGKKMSEDLP YEVRRAQEIN HLFGPKDSED SYDIIFDLHN TTSNMGCTLI LEDSRNNFLI QMFHYIKTSL APLPCYVYLI EHPSLKYATT RSIAKYPVGI EVGPQPQGVL RADILDQMRK MIKHALDFIH HFNEGKEFPP CAIEVYKIIE KVDYPRDENG EIAAIIHPNL QDQDWKPLHP GDPMFLTLDG KTIPLGGDCT VYPVFVNEAA YYEKKEAFAK TTKLTLNAKS IRCCLH