Recombinant humaan ASPH-eiwit

ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aspartyl/asparaginyl beta-hydroxylase, AAH, BAH, CASQ2BP1, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, Peptide aspartate beta dioxygenase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.5 kDa (217aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

ASPH, Aspartyl/asparaginyl beta-hydroxylase, AAH, BAH, CASQ2BP1, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, Peptide aspartate beta dioxygenase, ATGP0484-10 µg, ATGP0484-20 µg, ATGP0484-50 µg, ATGP0484-100 µg, ATGP0484-250 µg, ATGP0484-500 µg, ATGP0484-1 mg, ATGP0484-10, ATGP0484-20, ATGP0484-50, ATGP0484-100, ATGP0484-250, ATGP0484-500, ATGP0484-1

Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390 (4) :1389-94.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Redox Proteins

NP_001158227.1

Q12797

Human

FDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT