Recombinant humaan ASPRV1-eiwit

ASPRV1, also known as MuNO, SASP and ASAPase, is a protein that contains 1 peptidase A2 domain. This protein is expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles and localized to membrane region. This protein undergoes autocleavage which is necessary for activation of the protein. Recombinant human ASPRV1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aspartic peptidase retroviral-like 1, Aspartic peptidase, retroviral-like 1, MuNO, SASP, SASPase, Taps

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.2 kDa (159aa) confirmed by MALDI-TOF

ASPRV1, ATGP1768-10 µg, ATGP1768-20 µg, ATGP1768-50 µg, ATGP1768-100 µg, ATGP1768-250 µg, ATGP1768-500 µg, ATGP1768-1 mg, ATGP1768-10, ATGP1768-20, ATGP1768-50, ATGP1768-100, ATGP1768-250, ATGP1768-500, ATGP1768-1

Matsui, T., et al. (2011) EMBO Mol Med 3 (6), 320-333 ; ; Rhiemeier, V., et al. (2006) Am. J. Pathol. 168 (4), 1354-1364

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Enzymes & Proteases

NP_690005

Q53RT3

Human

SMGKGYY LKGKIGKVPV RFLVDSGAQV SVVHPNLWEE VTDGDLDTLQ PFENVVKVAN GAEMKILGVW DTAVSLGKLK LKAQFLVANA SAEEAIIGTD VLQDHNAILD FEHRTCTLKG KKFRLLPVGG SLEDEFDLE