Recombinant humaan ASPSCR1-eiwit

ASPSCR1 contains a uBX domain and interacts with glucose transporter type 4 (GLuT4) . This protein is a tether, which sequesters the GLuT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLuT4 to the plasma membrane within minutes of insulin stimulation. Translocation t (X;17) (p11; q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Recombinant human ASPSCR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tether containing uBX domain for GLuT4 isoform 1, ASPCR1, ASPL, ASPS, RCC17, TuG, uBXD9, uBXN9

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

62.6 kDa (576aa)

ASPSCR1, Tether containing uBX domain for GLuT4 isoform 1, ASPCR1, ASPL, ASPS, RCC17, TuG, uBXD9, uBXN9, ATGP2165-10 µg, ATGP2165-20 µg, ATGP2165-50 µg, ATGP2165-100 µg, ATGP2165-250 µg, ATGP2165-500 µg, ATGP2165-1 mg, ATGP2165-10, ATGP2165-20, ATGP2165-50, ATGP2165-100, ATGP2165-250, ATGP2165-500, ATGP2165-1

Bogan JS, Hendon N, et al. (2003) . Nature. 425 (6959) :727-33.; ; Argani P, Antonescu CR, et al. (2001) . Am J Pathol. 159 (1) :179-92.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Signal Transduction

NP_076988.1

Q9BZE9

Human

MAAPAGG GGSAVSVLAP NGRRHTVKVT PSTVLLQVLE DTCRRQDFNP CEYDLKFQRS VLDLSLQWRF ANLPNNAKLE MVPASRSREG PENMVRIALQ LDDGSRLQDS FCSGQTLWEL LSHFPQIREC LQHPGGATPV CVYTRDEVTG EAALRGTTLQ SLGLTGGSAT IRFVMKCYDP VGKTPGSLGS SASAGQAAAS APLPLESGEL SRGDLSRPED ADTSGPCCEH TQEKQSTRAP AAAPFVPFSG GGQRLGGPPG PTRPLTSSSA KLPKSLSSPG GPSKPKKSKS GQDPQQEQEQ ERERDPQQEQ ERERPVDREP VDREPVVCHP DLEERLQAWP AELPDEFFEL TVDDVRRRLA QLKSERKRLE EAPLVTKAFR EAQIKEKLER YPKVALRVLF PDRYVLQGFF RPSETVGDLR DFVRSHLGNP ELSFYLFITP PKTVLDDHTQ TLFQANLFPA ALVHLGAEEP AGVYLEPGLL EHAISPSAAD VLVARYMSRA AGSPSPLPAP DPAPKSEPAA EEGALVPPEP IPGTAQPVKR SLGKVPKWLK LPASKR