Recombinant humaan ASS1-eiwit

The ASS1 is an enzyme called argininosuccinate synthase 1. This enzyme participates in the urea cycle, which is a sequence of chemical reactions that takes place in liver cells. The urea cycle processes excess nitrogen that is generated as the body uses proteins. The excess nitrogen is used to make a compound called urea, which is excreted from the body in urine. Recombinant human ASS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

48.6 kDa (432aa) confirmed by MALDI-TOF

ASS1, Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase, ATGP0636-10 µg, ATGP0636-20 µg, ATGP0636-50 µg, ATGP0636-100 µg, ATGP0636-250 µg, ATGP0636-500 µg, ATGP0636-1 mg, ATGP0636-10, ATGP0636-20, ATGP0636-50, ATGP0636-100, ATGP0636-250, ATGP0636-500, ATGP0636-1

Kobayashi K, . et al. (1990) J Biol Chem. 265 (19) :11361-7

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_000041

P00966

Human

MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDVSREFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQVRFEL SCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DILEIEFKKG VPVKVTNVKD GTTHQTSLEL FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVLKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPTDATG FININSLRLK EYHRLQSKVT AK