Recombinant humaan AST1/GOT1-eiwit

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Aspartate transaminase 1, AST1, SGOT, AST, cAspAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase, Cytoplasmic, cCAT, Transaminase A

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

48.4 kDa (433aa) confirmed by MALDI-TOF

GOT1, Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18, ATGP3177-10 µg, ATGP3177-20 µg, ATGP3177-50 µg, ATGP3177-100 µg, ATGP3177-250 µg, ATGP3177-500 µg, ATGP3177-1 mg, ATGP3177-10, ATGP3177-20, ATGP3177-50, ATGP3177-100, ATGP3177-250, ATGP3177-500, ATGP3177-1

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22) : 5293-9.; ; Totan A., et al. (2006) Clin Chem Lab Med. 44 (5) :612-5.

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020) {https://pubmed.ncbi.nlm.nih.gov/31787239/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Metabolism

NP_002070

P17174

Human

MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ