Recombinant humaan Ataxine-3-eiwit

Ataxin 3 is also known as Machado-Joseph disease protein 1. Machado-Joseph disease is one of several hereditary autosomal dominant neurodegenerative disorders. This protein contains trinucleotide CAG repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. Ataxin 3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB) -binding protein, p300, and p300/CREB-binding protein-associated factor and inhibits transcription by these coactivators. Also, ataxin-3 is a proteasome-associated factor that mediates the degradation of ubiquitinated proteins. Recombinant human Ataxin-3 was expressed in E. coli and purified by using conventional chromatography.

Ataxin 3, ATX3, MJD, MJD1, SCA3, JOS, ATXN3, AT3, EC 3.4.22., Josephin, Machado Joseph disease, Machado Joseph disease protein 1, Machado-Joseph disease protein 1 homolog, MJD gene, Rsca3, SCA3 gene, Spinocerebellar ataxia type 3 protein

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

42.4 kDa (370aa) confirmed by MALDI-TOF

Ataxin3, Ataxin 3, ATX3, MJD, MJD1, SCA3, ATXN3, JOS, ATXN3, AT3, EC 3.4.22., Josephin, Machado Joseph disease, Machado Joseph disease protein 1, Machado-Joseph disease protein 1 homolog, MJD gene, Rsca3, SCA3 gene, Spinocerebellar ataxia type 3 protein, ATGP0344-10 µg, ATGP0344-20 µg, ATGP0344-50 µg, ATGP0344-100 µg, ATGP0344-250 µg, ATGP0344-500 µg, ATGP0344-1 mg, ATGP0344-10, ATGP0344-20, ATGP0344-50, ATGP0344-100, ATGP0344-250, ATGP0344-500, ATGP0344-1

Doss Pepe EW., et al. (2003) . Mol Cell Biol. 23 (18) :6469-83. ; ; Paulson., et al. (1997) . Ann Neurol. 41 (4) :453-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 50 mM NaCl, 10% glycerol

Epigenomics (Transcription & Translation)

AAH33711

P54252

Human

MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IRVQQMHRPK LIGEELAQLK EQRVHKTDLE RVLEANDGSG MLDEDEEDLQ RALALSRQEI DMEDEEADLR RAIQLSMQGS SRNISQDMTQ TSGTNLTSEE LRKRREAYFE KQQQKQQQQQ QQQQQQQQQQ QQQQGDLSGQ SSHPCERPAT SSGALGSDLG DAMSEEDMLQ AAVTMSLETV RNDLKTEGKK