Recombinant humaan ATF1-eiwit

Activating transcription factor 1, also known as ATF1, is a cyclic-AMP dependent transcription factor. ATF1 is expressed in a wide variety of cell types and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factor induction of c-jun expression requires ATF1 and MEF2 sites in the c-jun promoter. Recombinant human ATF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Activating transcription factor 1, EWS-ATF1, FuS/ATF-1, TREB36

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.8 kDa (295aa) confirmed by MALDI-TOF

ATF1, Activating transcription factor 1, EWS-ATF1, FuS/ATF-1, TREB36, ATGP1374-10 µg, ATGP1374-20 µg, ATGP1374-50 µg, ATGP1374-100 µg, ATGP1374-250 µg, ATGP1374-500 µg, ATGP1374-1 mg, ATGP1374-10, ATGP1374-20, ATGP1374-50, ATGP1374-100, ATGP1374-250, ATGP1374-500, ATGP1374-1

Lin Y S., et al. (1988) Proc Natl Acad Sci uSA. 85:3396-3400.; ; Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA

Signal Transduction

NP_005162.1

P18846

Human

MEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSD SIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMS VPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQ VVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKE YVKCLENRVA VLENQNKTLI EELKTLKDLY SNKSV