Recombinant humaan ATG10-eiwit

Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.7 kDa (243aa) confirmed by MALDI-TOF

ATG10, ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616, ATGP1742-10 µg, ATGP1742-20 µg, ATGP1742-50 µg, ATGP1742-100 µg, ATGP1742-250 µg, ATGP1742-500 µg, ATGP1742-1 mg, ATGP1742-10, ATGP1742-20, ATGP1742-50, ATGP1742-100, ATGP1742-250, ATGP1742-500, ATGP1742-1

Nemoto T., et al. (2003) J Bio Chem278:39517-39526; ; Shin J H., et al. (2009) Mol Cells. 27: 37-74.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Ubiquitination

NP_113670

Q9H0Y0

Human

MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP