Recombinant humaan ATG3-eiwit

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy. Recombinant human ATG3 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.3 kDa (337aa) confirmed by MALDI-TOF

ATG3, ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96, ATGP2715-10 µg, ATGP2715-20 µg, ATGP2715-50 µg, ATGP2715-100 µg, ATGP2715-250 µg, ATGP2715-500 µg, ATGP2715-1 mg, ATGP2715-10, ATGP2715-20, ATGP2715-50, ATGP2715-100, ATGP2715-250, ATGP2715-500, ATGP2715-1

Tanida I., et al. (2002) J Biol Chem. 277: 13739-13744.; ; Yamada Y., et al. (2007) J Biol Chem. 282: 8036-8043

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Ubiquitination

NP_071933

Q9NT62

Human

MGSSHHHHHH SSGLVPRGSH MGSMQNVINT VKGKALEVAE YLTPVLKESK FKETGVITPE EFVAAGDHLV HHCPTWQWAT GEELKVKAYL PTGKQFLVTK NVPCYKRCKQ MEYSDELEAI IEEDDGDGGW VDTYHNTGIT GITEAVKEIT LENKDNIRLQ DCSALCEEEE DEDEGEAADM EEYEESGLLE TDEATLDTRK IVEACKAKTD AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEQRQPL TVEHMYEDIS QDHVKKTVTI ENHPHLPPPP MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM