Recombinant humaan ATG4B-eiwit

ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Cysteine protease ATG4B, AuTL1, Autophagin-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.3 kDa (401aa) confirmed by MALDI-TOF

ATG4B, Cysteine protease ATG4B, AuTL1, Autophagin-1, ATGP1431-10 µg, ATGP1431-20 µg, ATGP1431-50 µg, ATGP1431-100 µg, ATGP1431-250 µg, ATGP1431-500 µg, ATGP1431-1 mg, ATGP1431-10, ATGP1431-20, ATGP1431-50, ATGP1431-100, ATGP1431-250, ATGP1431-500, ATGP1431-1

Sugawara K., et al. (2005) J. Biol. Chem. 280:40058-40065; ; Kumanomidou T., et al. (2006) J. Mol. Biol. 355:612-618;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF

Ubiquitination

AAH00719.1

Q9Y4P1

Human