Recombinant humaan ATG5-eiwit

Autophagy protein 5, also known as ATG5, belongs to the autophagy-related (ATG) genes family. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. ATG5 protein is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Recombinant human ATG5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Autophagy protein 5, APG5, APG5-LIKE, APG5L, ASP, hAPG5

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.6 kDa (295aa) confirmed by MALDI-TOF

ATG5, Autophagy protein 5, APG5, APG5-LIKE, APG5L, ASP, hAPG5, ATGP0903-10 µg, ATGP0903-20 µg, ATGP0903-50 µg, ATGP0903-100 µg, ATGP0903-250 µg, ATGP0903-500 µg, ATGP0903-1 mg, ATGP0903-10, ATGP0903-20, ATGP0903-50, ATGP0903-100, ATGP0903-250, ATGP0903-500, ATGP0903-1

Mizushima N., et al. (1998) Nature. 395:395-8.; ; Tanida I., et al. (1999) Mol Biol Cell. 10:1367-79.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Apoptosis

NP_004840

Q9H1Y0

Human

MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLPRVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNITVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLKEVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD