Recombinant humaan ATP1B1-eiwit

ATP1B1, also known as ATPase Na+/K+ transporting subunit beta 1, belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Recombinant human ATP1B1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 3,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25C.

ATPase Na+/K+ transporting subunit beta 1, ATP1B1, ATP1B

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 3,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25C.

29kDa (250aa)

ATP1B1, ATPase Na+/K+ transporting subunit beta 1, ATP1B1, ATP1B, ATGP3507-10 µg, ATGP3507-20 µg, ATGP3507-50 µg, ATGP3507-100 µg, ATGP3507-250 µg, ATGP3507-500 µg, ATGP3507-1 mg, ATGP3507-10, ATGP3507-20, ATGP3507-50, ATGP3507-100, ATGP3507-250, ATGP3507-500, ATGP3507-1

Selvakumar P. et al., (2014) Pathol. Int. 9:579-586. ; ; Shi JL. et al., (2016) Oncotarget. 19:2585-2595.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001668.1

P05026

Human

Ala

EFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKS