Recombinant humaan ATP1B3-eiwit

ATP1B3 belongs to the X (+) /potassium ATPases subunit beta family. This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Recombinant human ATP1B3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sodium/potassium-transporting ATPase subunit beta-3, ATPB-3, CD298

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

27.4 kDa (246aa) confirmed by MALDI-TOF

ATP1B3, sodium/potassium-transporting ATPase subunit beta-3, ATPB-3, CD298, ATGP2677-10 µg, ATGP2677-20 µg, ATGP2677-50 µg, ATGP2677-100 µg, ATGP2677-250 µg, ATGP2677-500 µg, ATGP2677-1 mg, ATGP2677-10, ATGP2677-20, ATGP2677-50, ATGP2677-100, ATGP2677-250, ATGP2677-500, ATGP2677-1

Malik N., et al (1998) . Mamm. Genome 9:136-143; ; Chi A., et al (2006) . J. Proteome Res. 5:3135-3144;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT

Metabolism

NP_001670

P54709

Human

MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA