Recombinant humaan ATP5D-eiwit

ATP5D, also known as F-ATPase delta subunit, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This protein is the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. Recombinant human ATP5D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

ATP synthase subunit delta mitochondrial, ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.3 kDa (167aa) confirmed by MALDI-TOF

ATP5D, ATP synthase subunit delta mitochondrial, ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATGP1099-10 µg, ATGP1099-20 µg, ATGP1099-50 µg, ATGP1099-100 µg, ATGP1099-250 µg, ATGP1099-500 µg, ATGP1099-1 mg, ATGP1099-10, ATGP1099-20, ATGP1099-50, ATGP1099-100, ATGP1099-250, ATGP1099-500, ATGP1099-1

Jordan E.M., et al. (1992) Biochim. Biophys. Acta 1130:123-126; ; Grimwood J., et al. (2004) Nature. 428:529-535

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Metabolism

NP_001678

P30049

Human

MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA HVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE