Recombinant humaan ATP5F1-eiwit

ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . Recombinant human ATP5F1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

ATP synthase subunit b, PIG47

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

22.6 kDa (197aa)

ATP5F1, ATP synthase subunit b, PIG47, ATGP2516-10 µg, ATGP2516-20 µg, ATGP2516-50 µg, ATGP2516-100 µg, ATGP2516-250 µg, ATGP2516-500 µg, ATGP2516-1 mg, ATGP2516-10, ATGP2516-20, ATGP2516-50, ATGP2516-100, ATGP2516-250, ATGP2516-500, ATGP2516-1

Higuti T., et al. (1991) Biochem. Biophys. Res. Commun. 178:1014-1020; ; Choudhary C., et al. (2009) Science. 325:834-840;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_001679

P24539

Human

MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM