Recombinant humaan ATP5H-eiwit

ATP synthase subunit d, also known as ATP5H, is a 161 amino acid protein that belongs to the ATPase d subunit family. ATP5H encodes the d subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane, ATP5H exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 17q25. 1. Recombinant human ATP5H protein, fused to His-tag at N-terminus, was expressed in E. coli.

ATP synthase subunit d mitochondrial isoform a, ATP synthase subunit d, mitochondrial isoform a, ATPQ

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.9 kDa (184aa)

ATP5H, ATP synthase subunit d mitochondrial isoform a, ATP synthase subunit d, mitochondrial isoform a, ATPQ, ATGP2248-10 µg, ATGP2248-20 µg, ATGP2248-50 µg, ATGP2248-100 µg, ATGP2248-250 µg, ATGP2248-500 µg, ATGP2248-1 mg, ATGP2248-10, ATGP2248-20, ATGP2248-50, ATGP2248-100, ATGP2248-250, ATGP2248-500, ATGP2248-1

Noh H S., et al. (2004) Brain Res. 129:80-87.; ; Sansanwal P., et al. (2010) J Am Soc Nephrol. 21:272-283.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_006347

O75947

Human

MAGRKLA LKTIDWVAFA EIIPQNQKAI ASSLKSWNET LTSRLAALPE NPPAIDWAYY KANVAKAGLV DDFEKKFNAL KVPVPEDKYT AQVDAEEKED VKSCAEWVSL SKARIVEYEK EMEKMKNLIP FDQMTIEDLN EAFPETKLDK KKYPYWPHQP IENL