Recombinant humaan ATP5O-eiwit

ATP synthase subunit O, also known as ATP5O, localizes to the mitochondria and catalyzes ATP synthesis. The protein is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. Recombinant human ATP5O protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ATP synthase subunit O, ATPO, OSCP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.1 kDa (211aa) confirmed by MALDI-TOF

ATP5O, ATP synthase subunit O, ATPO, OSCP, ATGP0847-10 µg, ATGP0847-20 µg, ATGP0847-50 µg, ATGP0847-100 µg, ATGP0847-250 µg, ATGP0847-500 µg, ATGP0847-1 mg, ATGP0847-10, ATGP0847-20, ATGP0847-50, ATGP0847-100, ATGP0847-250, ATGP0847-500, ATGP0847-1

Hundal T., et al. (1984) J Bioenerq Biomembr. 16:535-550.; ; Joshi S., et al. (1992) J Biol Chem. 267:12860-12867.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Signal Transduction

NP_001688

P48047

Human

FAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V