Recombinant humaan ATP6V1F-eiwit

ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. Recombinant human ATP6V1F, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

V-type proton ATPase subunit F, V-type proton ATPase subunit F, ATP6S14, VATF, Vma7

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.8 kDa (142aa) confirmed by MALDI-TOF

ATP6V1F, V-type proton ATPase subunit F, V-type proton ATPase subunit F, ATP6S14, VATF, Vma7, ATGP2947-10 µg, ATGP2947-20 µg, ATGP2947-50 µg, ATGP2947-100 µg, ATGP2947-250 µg, ATGP2947-500 µg, ATGP2947-1 mg, ATGP2947-10, ATGP2947-20, ATGP2947-50, ATGP2947-100, ATGP2947-250, ATGP2947-500, ATGP2947-1

Supino R., et al. (2009) Ann. N. Y. Acad. Sci. 1171, 606-616; ; Smith AN., et al. (2008) J. Bioenerg. Biomembr. 40 (4), 371-380;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 50% glycerol, 1mM DTT

Metabolism

NP_004222

Q16864

Human

MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR