Recombinant humaan ATPIF1-eiwit

ATPase inhibitory factor 1, also known as ATPIF1, binds to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, via interference of the beta and gamma subunit interaction, ATPIF1 regulates the activity of the F1 (the hydrophilic catalytic core), F0 (the membrane embedded protein channel) ATPase. The overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis. Recombinant human ATPIF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ATPase inhibitor mitochondrial, ATPI, ATPIP, IP, ATPase Inhibitory Factor 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

12.2 kDa (106aa) confirmed by MALDI-TOF

ATPIF1, ATPase inhibitor mitochondrial, ATPI, ATPIP, IP, ATPase Inhibitory Factor 1, ATGP1630-10 µg, ATGP1630-20 µg, ATGP1630-50 µg, ATGP1630-100 µg, ATGP1630-250 µg, ATGP1630-500 µg, ATGP1630-1 mg, ATGP1630-10, ATGP1630-20, ATGP1630-50, ATGP1630-100, ATGP1630-250, ATGP1630-500, ATGP1630-1

Sanchez Cenizo L., et al. (2010) J Biol Chem. 285: 25308-13.; ; Cortes Hernandez P., et al. (2005) Biochem Biophys Res Commun. 330: 844-849.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol

Metabolism

NP_057395.1

Q9UII2

Human

GSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD