Recombinant humaan AUH-eiwit

AUH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity. Recombinant human AUH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

AU RNA binding methylglutaconyl-CoA hydratase, AU RNA binding protein/enoyl-Coenzyme A hydratase, AU-specific RNA-binding enoyl-CoA hydratase, Methylglutaconyl-CoA hydratase mitochondrial, 3-MG-CoA hydratase, Itaconyl-CoA hydratase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

31.4 kDa (293aa) confirmed by MALDI-TOF

Methylglutaconyl-CoA hydratase mitochondrial, Itaconyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, AU specific RNA binding enoyl CoA hydratase, AU RNA binding protein/enoyl-Coenzyme A hydratase, AU RNA binding methylglutaconyl-CoA hydratase, 3-MG-CoA hydratase, 3 MG CoA hydratase, Methylglutaconyl CoA hydratase mitochondrial, Itaconyl CoA hydratase, ATGP0838-10 µg, ATGP0838-20 µg, ATGP0838-50 µg, ATGP0838-100 µg, ATGP0838-250 µg, ATGP0838-500 µg, ATGP0838-1 mg, ATGP0838-010, ATGP0838-020, ATGP0838-050, ATGP0838-100, ATGP0838-250, ATGP0838-500, ATGP0838-01M

Kurimoto K, et al. (2009) . Proteins 75 (2) :360-72; ; Mack M., et al. (2006) . FEBS J. 273 (9) :2012-22

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Metabolism

NP_001689

Q13825

Human

MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE