Recombinant humaan Aurora A-eiwit

Aurora Kinase A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora kinase A is highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also this protein plays a crucial role in tumourigenesis and is overexpressed in many kinds of cancers. Recombinant human Aurora-A fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Serine/threonine-protein kinase 6, Aurora-A, AIK, ARK1, AuRA, BTAK, STK15, STK6, Aurora kinase A, AuRKA, Serine/threonine-protein kinase 6 Aurora 2, Aurora A, aurora kinase A, IAK, Serine/threonine kinase 15.

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

47.9 kDa (423aa)

Aurora A, Serine/threonine-protein kinase 6, Aurora-A, AIK, ARK1, AuRA, BTAK, STK15, STK6, Aurora kinase A, AuRKA, Serine/threonine-protein kinase 6 Aurora 2, Aurora A, aurora kinase A, IAK, Serine/threonine kinase 15., ATGP0343-10 µg, ATGP0343-20 µg, ATGP0343-50 µg, ATGP0343-100 µg, ATGP0343-250 µg, ATGP0343-500 µg, ATGP0343-1 mg, ATGP0343-10, ATGP0343-20, ATGP0343-50, ATGP0343-100, ATGP0343-250, ATGP0343-500, ATGP0343-1

Tchatchou S., et al. (2007) . Cancer lett. 247 (2) : 266-72; ; Chen SS., et al. (2002) . EMBO J. 21 (17) : 4491-9;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 100 mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF, 20% glycerol

Cell Cycle

NP_003591.2

O14965

Human

MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS