Recombinant humaan B-Raf-eiwit

BRAF belongs to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. Recombinant human BRAF protein, fused to His-tag at N-terminus, was expressed in E. coli.

Serine/threonine-protein kinase B-raf, Serine/threonine-protein kinase B-raf, B-RAF1, BRAF1, NS7, RAFB1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

40.6 kDa (360aa)

B-Raf, Serine/threonine-protein kinase B-raf, Serine/threonine-protein kinase B-raf, B-RAF1, BRAF1, NS7, RAFB1, ATGP2924-10 µg, ATGP2924-20 µg, ATGP2924-50 µg, ATGP2924-100 µg, ATGP2924-250 µg, ATGP2924-500 µg, ATGP2924-1 mg, ATGP2924-10, ATGP2924-20, ATGP2924-50, ATGP2924-100, ATGP2924-250, ATGP2924-500, ATGP2924-1

Brennan D.F., et al. (2011) Nature 472:366-369

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Apoptosis

NP_004324.2

P15056

Human

SEDRN RMKTLGRRDS SDDWEIPDGQ ITVGQRIGSG SFGTVYKGKW HGDVAVKMLN VTAPTPQQLQ AFKNEVGVLR KTRHVNILLF MGYSTKPQLA IVTQWCEGSS LYHHLHIIET KFEMIKLIDI ARQTAQGMDY LHAKSIIHRD LKSNNIFLHE DLTVKIGDFG LATVKSRWSG SHQFEQLSGS ILWMAPEVIR MQDKNPYSFQ SDVYAFGIVL YELMTGQLPY SNINNRDQII FMVGRGYLSP DLSKVRSNCP KAMKRLMAEC LKKKRDERPL FPQILASIEL LARSLPKIHR SASEPSLNRA GFQTEDFSLY ACASPKTPIQ AGGYGAFPVH