Recombinant humaan B3GAT1-eiwit

B3GAT1, also known as galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, is a key enzyme during the biosynthesis of the carbohydrate epitope HNK-1. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This protein is expressed as a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Also, the enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. This protein functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1. Recombinant Human B3GAT1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

62.3 kDa (549aa)

B3GAT1, ATGP3892-10 µg, ATGP3892-20 µg, ATGP3892-50 µg, ATGP3892-100 µg, ATGP3892-250 µg, ATGP3892-500 µg, ATGP3892-1 mg, ATGP3892-10, ATGP3892-20, ATGP3892-50, ATGP3892-100, ATGP3892-250, ATGP3892-500, ATGP3892-1, ATGP3892-10 µg, ATGP3892-20 µg, ATGP3892-50 µg, ATGP3892-100 µg, ATGP3892-250 µg, ATGP3892-500 µg, ATGP3892-1 mg, ATGP3892-10, ATGP3892-20, ATGP3892-50, ATGP3892-100, ATGP3892-250, ATGP3892-500, ATGP3892-1

Terayama K., et al, (1997) Proc. Natl. Acad. Sci. USA 94:6093-6098.; ; Kakuda, S., et al, (2005) Glycobiology 2:203-210.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol

Immunology

NP_473366

Q9P2W7

Human

Ala

TLAPLLA VHKDEGSDPR RETPPGADPR EYCTSDRDIV EVVRTEYVYT RPPPWSDTLP TIHVVTPTYS RPVQKAELTR MANTLLHVPN LHWLVVEDAP RRTPLTARLL RDTGLNYTHL HVETPRNYKL RGDARDPRIP RGTMQRNLAL RWLRETFPRN SSQPGVVYFA DDDNTYSLEL FEEMRSTRRV SVWPVAFVGG LRYEAPRVNG AGKVVGWKTV FDPHRPFAID MAGFAVNLRL ILQRSQAYFK LRGVKGGYQE SSLLRELVTL NDLEPKAANC TKILVWHTRT EKPVLVNEGK KGFTDPSVEI