Recombinant humaan B3GAT3-eiwit

Beta-1, 3-glucuronyltransferase 3, also known as B3GAT3, is involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. This protein can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. It shows strict specificity for Gal-beta-1, 3-Gal-beta-1, 4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Recombinant human B3GAT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Beta-13-glucuronyltransferase 3, Beta-1,3-glucuronyltransferase 3, GLCATI

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.4 kDa (330aa) confirmed by MALDI-TOF

B3GAT3, Beta-13-glucuronyltransferase 3, Beta-1,3-glucuronyltransferase 3, GLCATI, ATGP2114-10 µg, ATGP2114-20 µg, ATGP2114-50 µg, ATGP2114-100 µg, ATGP2114-250 µg, ATGP2114-500 µg, ATGP2114-1 mg, ATGP2114-10, ATGP2114-20, ATGP2114-50, ATGP2114-100, ATGP2114-250, ATGP2114-500, ATGP2114-1

Pedersen L.C., et al. (2000) J. Biol. Chem. 275:34580-34585; ; Tone Y., et al. (2008) J. Biol. Chem. 283:16801-16807

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_036332

O94766

Human

MGSSHHHHHH SSGLVPRGSH MGSQPCDCLP PLRAAAEQLR QKDLRISQLQ AELRRPPPAP AQPPEPEALP TIYVVTPTYA RLVQKAELVR LSQTLSLVPR LHWLLVEDAE GPTPLVSGLL AASGLLFTHL VVLTPKAQRL REGEPGWVHP RGVEQRNKAL DWLRGRGGAV GGEKDPPPPG TQGVVYFADD DNTYSRELFE EMRWTRGVSV WPVGLVGGLR FEGPQVQDGR VVGFHTAWEP SRPFPVDMAG FAVALPLLLD KPNAQFDSTA PRGHLESSLL SHLVDPKDLE PRAANCTRVL VWHTRTEKPK MKQEEQLQRQ GRGSDPAIEV