Recombinant humaan B7-1/CD80-eiwit

CD80, also known as T-lymphocyte activation antigen CD80, is a member of cell surface immunoglobulin superfamily. This protein can bind to either human or mouse CD28 and CTLA-4. It is expressed on activated B cells, activated T cells, and macrophages. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. Therefore, it is considered a promising therapeutic target for autoimmune diseases and various carcinomas. Recombinant human CD80, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CD80 is coated at 1 µg/mL (100 ul/well) can bind CD28 (CAT# ATGP3603) in a Functional ELISA assay.

Activation B7-1 antigen, B7, B7.1, B7-1, BB1, CD28LG, CD28LG1, CD80, CTLA-4 counter-receptor B7.1, FcalphaRI, LAB7, T-lymphocyte activation antigen CD80

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human CD28 (CAT# ATGP3603)

24.9 kDa (216aa)

B71, CD80, T-lymphocyte activation antigen CD80, CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7, ATGP3663-10 µg, ATGP3663-20 µg, ATGP3663-50 µg, ATGP3663-100 µg, ATGP3663-250 µg, ATGP3663-500 µg, ATGP3663-1 mg, ATGP3663-10, ATGP3663-20, ATGP3663-50, ATGP3663-100, ATGP3663-250, ATGP3663-500, ATGP3663-1

Zang X., et al. (2007) Clin Cancer Res. 13:5271-5279.; ; Kakoulidou M., et al. (2007) Scand. J. Immunol. 66:529-537.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_005182

P33681

Human

Val35