Recombinant humaan B7-2/CD86-eiwit

B7-2/CD86, also known as T-lymphocyte activation antigen CD86 isoform 1, is a variably glycosylated protein in the B7 family. These family members play important roles in immune activation and the maintenance of immune tolerance. It is expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival and exists predominantly as a monomer on cell surfaces. Also, it interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells that it induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. Recombinant Human B7-2/CD86, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

B7-2/CD86, CD86, B7-2, B7.2, B70, CD28LG2, LAB72, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

25.6 kDa (223aa)

B72, CD86, ATGP3895-10 µg, ATGP3895-20 µg, ATGP3895-50 µg, ATGP3895-100 µg, ATGP3895-250 µg, ATGP3895-500 µg, ATGP3895-1 mg, ATGP3895-10, ATGP3895-20, ATGP3895-50, ATGP3895-100, ATGP3895-250, ATGP3895-500, ATGP3895-1, ATGP3895-10 µg, ATGP3895-20 µg, ATGP3895-50 µg, ATGP3895-100 µg, ATGP3895-250 µg, ATGP3895-500 µg, ATGP3895-1 mg, ATGP3895-10, ATGP3895-20, ATGP3895-50, ATGP3895-100, ATGP3895-250, ATGP3895-500, ATGP3895-1

Grujic M., et al, (2010) Journal of Immunology. 185 (3) : 1730-1743.; ; Kuchroo VK., et al, (1995) Cell 80:707-718.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_787058

P42081

Human

Tyr31