Recombinant humaan B7-H4-eiwit

VTCN1, also known as v-set domain-containing T-cell activation inhibitor 1 isoform 1, is a member of the costimulatory B7 family. It has been found to be widely expressed in many kinds of tumor tissues and to play an important part in tumor progression and poor prognosis. It serves a critical role in the negative regulation of T cell-mediated antitumor immune responses. It facilitates proliferation of esophageal squamous cell carcinoma cells through promoting interleukin-6/signal transducer and activator of transcription 3 pathway activation. Recombinant human VTCN1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

V-set domain-containing T-cell activation inhibitor 1 isoform 1, VTCN1, B7-H4, B7h.5, B7H4, B7S1, B7X, PRO1291

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

26.9 kDa (244aa)

B7-H4, V-set domain-containing T-cell activation inhibitor 1 isoform 1, VTCN1, B7-H4, B7h.5, B7H4, B7S1, B7X, PRO1291, ATGP3607-10 µg, ATGP3607-20 µg, ATGP3607-50 µg, ATGP3607-100 µg, ATGP3607-250 µg, ATGP3607-500 µg, ATGP3607-1 mg, ATGP3607-10, ATGP3607-20, ATGP3607-50, ATGP3607-100, ATGP3607-250, ATGP3607-500, ATGP3607-1

Zeng S., et al. (2016) Mol Biol. 50:1007-1013.; ; Chen X., et al. (2016) Cancer Sci. 107:944-954.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_078902.2

Q7Z7D3

Human

Ala

LIIGFGI SGRHSITVTT VASAGNIGED GILSCTFEPD IKLSDIVIQW LKEGVLGLVH EFKEGKDELS EQDEMFRGRT AVFADQVIVG NASLRLKNVQ LTDAGTYKCY IITSKGKGNA NLEYKTGAFS MPEVNVDYNA SSETLRCEAP RWFPQPTVVW ASQVDQGANF SEVSNTSFEL NSENVTMKVV SVLYNVTINN TYSCMIENDI AKATGDIKVT ESEIKRRSHL QLLNSKAS