Recombinant humaan B9D2-eiwit

B9D2 contains a B9 domain protein, which are exclusively found in ciliated organisms. This protein is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects. Recombinant human B9D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

B9 domain-containing protein 2, ICIS-1, MKS10, MKSR2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

21.6 kDa (198aa) confirmed by MALDI-TOF

B9D2, B9 domain-containing protein 2, ICIS-1, MKS10, MKSR2, ATGP2768-10 µg, ATGP2768-20 µg, ATGP2768-50 µg, ATGP2768-100 µg, ATGP2768-250 µg, ATGP2768-500 µg, ATGP2768-1 mg, ATGP2768-10, ATGP2768-20, ATGP2768-50, ATGP2768-100, ATGP2768-250, ATGP2768-500, ATGP2768-1

Dowdle W.E., et al. (2011) Am. J. Hum. Genet. 89:94-110; ; Bialas N.J., et al. (2009) J. Cell Sci. 122:611-624

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Signal Transduction

NP_085055

Q9BPU9

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEVHVI GQIIGASGFS ESSLFCKWGI HTGAAWKLLS GVREGQTQVD TPQIGDMAYW SHPIDLHFAT KGLQGWPRLH FQVWSQDSFG RCQLAGYGFC HVPSSPGTHQ LACPTWRPLG SWREQLARAF VGGGPQLLHG DTIYSGADRY RLHTAAGGTV HLEIGLLLRN FDRYGVEC