Recombinant humaan BACE-1-eiwit

BACE-1, also known as beta-secretase 1 isoform A, is a member of aspartic protease and an integral membrane protein. It is involved in the proteolytic processing of the amyloid precursor protein (APP) . This protein cleaves at the N-terminus of the A-beta peptide sequence of APP, and then leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has been implicated in the onset and/or progression of Alzheimer's disease. It is also distantly related to the pathogenic aspartic-acid protease plasmepsin, which is a potential target for future anti-malarial drugs. Recombinant human BACE-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 5 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

BACE1, Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE, KIAA1149, HSPC104

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 5pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK (Dnp) RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

49.2 kDa (442aa)

BACE1, ATGP3917-10 µg, ATGP3917-20 µg, ATGP3917-50 µg, ATGP3917-100 µg, ATGP3917-250 µg, ATGP3917-500 µg, ATGP3917-1 mg, ATGP3917-10, ATGP3917-20, ATGP3917-50, ATGP3917-100, ATGP3917-250, ATGP3917-500, ATGP3917-1

Andrew RJ., et al. (2013) Proc Natl Acad Sci U S A. 114:E9665-E9674.; ; Brendel M., et al. (2018) Theranostics. 8:4957-4968.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Enzymes & Proteases

NP_036236

P56817

Human

Thr22