Recombinant humaan BBS3/ARL6-eiwit

ARL6, also known ADP-ribosylation factor-like protein 6, belongs to the ARF family of GTP-binding proteins. This protein is known to play essential roles in modulating membrane trafficking and cytoskeletal functions. Mutation in ARL6 causes Bardet-Biedl syndrome (BBS3) . BBS is a pleiotropic genetic disorder characterized by obesity, photoreceptor degeneration, polydactyly, hypogenitalism, renal abnor-malities and developmental delay. Recombinant human ARL6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

ADP-ribosylation factor-like protein 6, BBS3, MGC32934, RP55, ADP ribosylation factor like GTPase 6, Bardet-Biedl syndrome 3 protein

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.2 kDa (206aa) confirmed by MALDI-TOF

ARL6, ADP-ribosylation factor-like protein 6, BBS3, MGC32934, RP55, ATGP1084-10 µg, ATGP1084-20 µg, ATGP1084-50 µg, ATGP1084-100 µg, ATGP1084-250 µg, ATGP1084-500 µg, ATGP1084-1 mg, ATGP1084-10, ATGP1084-20, ATGP1084-50, ATGP1084-100, ATGP1084-250, ATGP1084-500, ATGP1084-1

Wiens CJ. et al. (2010) J Biol Chem. 285:16218-30.; ; Chiang AP. et al. (2004) Am J Hum Genet. 75: 475-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl,5mM DTT

Signal Transduction

NP_115522

Q9H0F7

Human

MGLLDRLSVL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQNILPT IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKEGQAI IFVIDSSDRL RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDAVTS VKVSQLLCLE NIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQTVKT