Recombinant humaan BCAT1-eiwit

BCAT1, also known as branched chain amino-acid transaminase, cytosolic, catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Recombinant human BCAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.4 kDa (409aa) confirmed by MALDI-TOF

BCAT1, Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18, ATGP1479-10 µg, ATGP1479-20 µg, ATGP1479-50 µg, ATGP1479-100 µg, ATGP1479-250 µg, ATGP1479-500 µg, ATGP1479-1 mg, ATGP1479-10, ATGP1479-20, ATGP1479-50, ATGP1479-100, ATGP1479-250, ATGP1479-500, ATGP1479-1

Goto M., et al. (2005) J. Biol. Chem. 280:37246-37256; ; Schuldiner O., et al. (1996) Proc. Natl. Acad. Sci. u.S.A. 93:7143-7148

Günther J, et al. BAY-069, a Novel (Trifluoromethyl) pyrimidinedione-Based BCAT1/2 Inhibitor and Chemical Probe. (J Med Chem. 2022) {https://pubmed.ncbi.nlm.nih.gov/36261130/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Metabolism

NP_005495

P54687

Human

MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS