Recombinant humaan BCKDHA-eiwit

BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) . Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

47.8 kDa (421aa) confirmed by MALDI-TOF

BCKDHA, 2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A, ATGP0944-10 µg, ATGP0944-20 µg, ATGP0944-50 µg, ATGP0944-100 µg, ATGP0944-250 µg, ATGP0944-500 µg, ATGP0944-1 mg, ATGP0944-10, ATGP0944-20, ATGP0944-50, ATGP0944-100, ATGP0944-250, ATGP0944-500, ATGP0944-1

Nobukuni Y., et al. (1993) Biochim. Biophys. Acta 1225:64-70; ; Funchal, C., et al. (2005) Metab. Brain Dis. 20: 205-217

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl

Enzymes & Proteases

NP_000700.1

P12694

Human

MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K