Recombinant humaan Bcl-10 eiwit

BCL10, also known as B-cell lymphoma/leukemia 10, contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. It is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Recombinant human BCL10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

B cell CLL/lymphoma 10, BCL10, Immune signaling adaptor, CARMEN, CIPER, mE10, c-E10, CLAP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.8 kDa (257aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

, B cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, BCL10, CARMEN, c-E10, CIPER, CLAP, IMD37, Immune signaling adaptor, mE10, B cell lymphoma/leukemia 10, c E10, Ce10, ATGP1457-10 µg, ATGP1457-20 µg, ATGP1457-50 µg, ATGP1457-100 µg, ATGP1457-250 µg, ATGP1457-500 µg, ATGP1457-1 mg, ATGP1457-010, ATGP1457-020, ATGP1457-050, ATGP1457-100, ATGP1457-250, ATGP1457-500, ATGP1457-01M

Rebeaud F., et al. (2008) Nat. Immunol. 9:272-281; ; Yui D., et al. (2001) Oncogene. 20:4317-4323

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.05M NaCl, 1mM DTT

Signal Transduction

NP_003912

O95999

Human

MEPTAP SLTEEDLTEV KKDALENLRV YLCEKIIAER HFDHLRAKKI LSREDTEEIS CRTSSRKRAG KLLDYLQENP KGLDTLVESI RREKTQNFLI QKITDEVLKL RNIKLEHLKG LKCSSCEPFP DGATNNLSRS NSDESNFSEK LRASTVMYHP EGESSTTPFF STNSSLNLPV LEVGRTENTI FSSTTLPRPG DPGAPPLPPD LQLEEEGTCA NSSEMFLPLR SRTVSRQ