Recombinant humaan BCL7A-eiwit

BCL7A is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human BCL7A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

B-cell CLL/lymphoma 7 protein family member A, BCL7

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.2 kDa (233aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

BCL7A, B-cell CLL/lymphoma 7 protein family member A, BCL7, ATGP1854-10 µg, ATGP1854-20 µg, ATGP1854-50 µg, ATGP1854-100 µg, ATGP1854-250 µg, ATGP1854-500 µg, ATGP1854-1 mg, ATGP1854-10, ATGP1854-20, ATGP1854-50, ATGP1854-100, ATGP1854-250, ATGP1854-500, ATGP1854-1

Carbone, A., et al. (2008) Genes Chromosomes Cancer 47 (12), 1067-1075; ; Morton, L.M., et al. (2009) Cancer Epidemiol. Biomarkers Prev. 18 (4), 1259-1270;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA

Metabolism

NP_001019979

Q4VC05

Human

MGSSHHHHHH SSGLVPRGSH MGSMSGRSVR AETRSRAKDD IKRVMAAIEK VRKWEKKWVT VGDTSLRIYK WVPVTEPKVD DKNKNKKKGK DEKCGSEVTT PENSSSPGMM DMHDDNSNQS SIADASPIKQ ENSSNSSPAP EPNSAVPSDG TEAKVDEAQA DGKEHPGAED ASDEQNSQSS MEHSMNSSEK VDRQPSGDSG LAAETSAISQ DLEGVPPSKK MKLEASQQNS EEM