Recombinant humaan BDH1-eiwit

BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R) -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.3 kDa (318aa) confirmed by MALDI-TOF

BDH1, D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1, ATGP1341-10 µg, ATGP1341-20 µg, ATGP1341-50 µg, ATGP1341-100 µg, ATGP1341-250 µg, ATGP1341-500 µg, ATGP1341-1 mg, ATGP1341-10, ATGP1341-20, ATGP1341-50, ATGP1341-100, ATGP1341-250, ATGP1341-500, ATGP1341-1

Marks A.R. et al. (1992) J Biol Chem. 267:15459-15463.; ; Adami P. et al. (1993) Biochem. J. 292: 863-872.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.2M NaCl,2mM DTT, 0.1mM PMSF

Metabolism

NP_976059

Q02338

Human

ASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVC SSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPAR SPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDT SPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR