Recombinant humaan BDH2-eiwit

BDH2, also known as DHRS6, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of BDH2 make it suitable to convert high levels of circulating 3-hydroxybutyrate into acetoacetate. Recombinant human BDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

3-hydroxybutyrate dehydrogenase type 2, DHRS6, EFA6R, FLJ13261, PRO20933, SDR15C1, uCPA-OR, uNQ6308

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.8 kDa (265aa) confirmed by MALDI-TOF

BDH2, 3-hydroxybutyrate dehydrogenase type 2, DHRS6, EFA6R, FLJ13261, PRO20933, SDR15C1, uCPA-OR, uNQ6308, ATGP0882-10 µg, ATGP0882-20 µg, ATGP0882-50 µg, ATGP0882-100 µg, ATGP0882-250 µg, ATGP0882-500 µg, ATGP0882-1 mg, ATGP0882-10, ATGP0882-20, ATGP0882-50, ATGP0882-100, ATGP0882-250, ATGP0882-500, ATGP0882-1

Guo K., et al. (2006) . J. Biol. Chem., 281: 10291-10297; ; Ito K., et al. (2006) . J. Mol. Biol., 355: 722-733.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Metabolism

NP_064524

Q9BUT1

Human

MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL