Recombinant humaan Becline 1/BECN1-eiwit

BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Beclin 1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, Beclin 1 autophagy related, ATG6, ATG6 autophagy related 6 homolog, VPS30

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

54.1 kDa (470aa)

Beclin1, beclin 1, ATG6, beclin1, VPS30, ATGP1816-10 µg, ATGP1816-20 µg, ATGP1816-50 µg, ATGP1816-100 µg, ATGP1816-250 µg, ATGP1816-500 µg, ATGP1816-1 mg, ATGP1816-10, ATGP1816-20, ATGP1816-50, ATGP1816-100, ATGP1816-250, ATGP1816-500, ATGP1816-1

Sagona A.P., et al. (2010) Nat. Cell Biol. 12:362-371; ; Itakura E., et al. (2008) Mol. Biol. Cell 19:5360-5372;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_003757.1

Q14457

Human

MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK